Fixatives to preserve flourescence

Graham Atherton grggta at picr.cr.man.ac.uk
Wed Mar 6 08:45:30 EST 1996


Can anyone give me an idea if GFP fluorescence can be maintained after
fixing the cells? 4%paraformaldehyde is supposed to be OK - are there any
others? I tried 4%PF on COS cells and saw no fluorescence - what parameters
are important for maintainance of fluorescence?
Thanks for any help
Graham



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