GFP and networking problems

[f.s.wouters]

Hi everybody,

for some mysterious reason, I'm able to post here but can't read in 
bionet.molbio.proteins.fluorescent. I'd be grateful if you could reply 
by mail. Maybe someone can tell me where I can find the FAQ?
anyway, my question is about the pGFP-N3 N-terminal protein fusion 
vector (Clontech). To put the problem simple: IT DOESN'T WORK. No 
matter what, lower temperatures, different celltypes....even the 
control doesn't work.
Who has the same (or even better: the opposite) results with the same 
vector and knows what the problem is (or even better: was).

any aditional information on GFP fusion protein systems are very 
welcome!

please use my mail address: petra at emsaserv.biol.ruu.nl , since I'm 
using somebody else's box right now (it's about time our network is 
fixed!)

Thanx a zillion in advance,


Petra de Graaf