fluorescence of GFP

Michael Moser moser at U.WASHINGTON.EDU
Mon Mar 18 11:13:11 EST 1996


Karen
	You can find the complete text of that message as well as all the
messages ever posted to this and every other biosci newsgroup by simply 
pointing your web brouser to the Biosci WWW server:

http://www.bio.net/

Select the link entitled:

"Access the BIOSCI/bionet Newsgroups"

Then link to the fluorescent proteins group.

A search of the archive with the word fluorimeter as a key yields two 
postings:

1.) yours
2.) one from misha at bragg.bio.purdue.edu

Happy GFPing,

Mike Moser                                             Tel: 206-543-5354
UW Department of Biochemistry                          FAX: 206-685-1792
Box 357350                                        moser at u.washington.edu
Seattle, WA  98195-7350             http://weber.u.washington.edu/~moser

On 18 Mar 1996, Karen P Scott wrote:

> Hi, A while ago someone wrote into this news group describing the minitoring
> of GFP fluorescence using a fluorimeter in a process which required washing
> the cells expessing the GFP with buffer prior to reading the fluorescence.
> this avoided problems of fluorescence of LB. Unfortunately our computer
> system crashed and I lost the details of the buffer which the person had
> kindly sent me. Thus I would be very grateful to receive the details again,
> and also the wavelenghts used for optimal excitation. (We were exciting at
> 395nm and monitoring the emission at 509nm).
> 
> Thanking you in advance, 
> Karen Scott.
> 
> 
> 



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