fluorescence of GFP
moser at U.WASHINGTON.EDU
Mon Mar 18 11:13:11 EST 1996
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A search of the archive with the word fluorimeter as a key yields two
2.) one from misha at bragg.bio.purdue.edu
Mike Moser Tel: 206-543-5354
UW Department of Biochemistry FAX: 206-685-1792
Box 357350 moser at u.washington.edu
Seattle, WA 98195-7350 http://weber.u.washington.edu/~moser
On 18 Mar 1996, Karen P Scott wrote:
> Hi, A while ago someone wrote into this news group describing the minitoring
> of GFP fluorescence using a fluorimeter in a process which required washing
> the cells expessing the GFP with buffer prior to reading the fluorescence.
> this avoided problems of fluorescence of LB. Unfortunately our computer
> system crashed and I lost the details of the buffer which the person had
> kindly sent me. Thus I would be very grateful to receive the details again,
> and also the wavelenghts used for optimal excitation. (We were exciting at
> 395nm and monitoring the emission at 509nm).
> Thanking you in advance,
> Karen Scott.
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