Re-"fluorescein bleaching"

Joachim Forsgren Joachim.Forsgren at
Wed May 15 04:24:51 EST 1996

To John McDonald: This isn't exactly chemical bleaching but could perhaps
be tried : I've used a mouse anti-FITC MAb from DAKOPATTS to detect FITC in
tissue sections where fluorescence had faded. The MAb also efficiently
quenches the fluorescence of "fresh" FITC. This also worked on a
FITC-conjugated DNA probe for in situ hybridization.

Sincerely, Joachim Forsgren, Karolinska institute, Sweden

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