Clontech pEGFP sequence ?

Paul Kitts pakitts at clontech.com
Fri May 24 13:45:05 EST 1996


> Are the sequences for the new pEGFP, pEGFP-N1, and pEGFP-C1 vectors 
> from Clontech available?  

These sequences were submitted to GenBank 5 weeks ago but they have 
not finished processing them yet. Even before GenBank releases the 
sequences, you can reqested them by sending an Email to 
tech at clontech.com.

> pEGFP is accession number U43284 with a F64L mutation.

As Gerard Rouwendal wrote, you can derive the sequence of the EGFP 
gene by adding the F64L mutation to the sequence of Seed's humanized 
GFP gene in phGFP-S65T (U43284). Note however, that the vector 
backbone of pEGFP-1, pEGFP-C1 & pEGFP-N1 is completely different from 
the phGFP-S65T backbone.

-= Paul =- 

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! Paul Kitts                                                !
! CLONTECH Laboratories, Inc.                               !
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