gfp and aequorin

Rees Jean-Francois Reesjf at bani.ucl.ac.be
Mon Nov 4 09:45:44 EST 1996


In article (Dans l'article)
<Pine.SUN.3.95L.961013100911.13962B-100000 at sawasdee.cc.columbia.edu>,
Xiaoxi  Zhuang <xz9 at columbia.edu> wrote (écrivait) :

> I'm very surprised to notice that there's only one
> paper on gfp transgenic mice and none on aequorin 
> transgenic mice.  I have been thinking
> about doing transgenic mice to express aequorin in
> brain, in order to study neuronal calcium activity 
> on brain slice. Will it be difficult to see it because 
> of incubation with coelentarazine, the expression
> level may be covered by endogenous fluorescence, also
> it may be difficult to have subcellular resolution
> on brain slice.   Anyway, what's the difficulty on 
> gfp transgenic mice?  Is gfp much stronger than
> aequorin? I will appreciate it if anyone could 
> give me some suggestions.
> 
> Xiaoxi

I would expect that aequorin will be more sensitive (backround
luminescence is very low whereas fluorescent materials could be present).
Coelenterazine could indeed produce some spontaneous luminescence with
oxygen or activated species but this should not pose major problems is
apoaequorin is produced at a sufficient level.



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