IP'ing GFP fusions

[Thomas Siegmund]

Marc Tritel wrote:
> 
> Does anybody know of an alternative to the Clontech anti-GFP antibody?
> We've tried to use it to immunoprecipitate GFP fusion proteins, and it is
> not very efficient in our hands. Much thanks to anyone who can help!

I have used a rabbit serum from Molecular Probes
(http://www.probes.com/) successfully in immunohistochemistry.

Thomas Siegmund
Institut fuer Genetik
Freie Universitaet Berlin