Linkers in GFP Fusions

GMEACHAM at bmg.bhs.uab.edu GMEACHAM at bmg.bhs.uab.edu
Thu Nov 14 14:11:19 EST 1996


I am tagging the N terminus of a cytostolic protein. Besides a frame 
shift, has anyone ran into trouble making a linker for a GFP tag, i.e. 
is length of the linker/amino acids encoded in linker problematic? 
Thanks.
G. Meacham
UAB Dept. of Cell Biology




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