General GFP in yeast

Peter Corish pcor at bioch.ox.ac.uk
Tue Sep 3 12:51:17 EST 1996


  We have a final year undergraduate student, who would like to use GFP in
S.cervisiae as a marker for chromosome stability.
   Does anybody have GFP(preferably S65T, but not essential)  expressed
from a good yeast promoter that gives a nice fluorescent signal.  It isn't
a problem if it is a fusion protein that is localised to a cell structure,
but if it is localised it must be easily seen without too powerful a
magnification (x20 or x40 objectives). 
   It would be much appreciated if you were willing to share this
construct with for the purposes of this project.
  Thanks alot
____________________________________________________________________________
Pete Corish,
CRC Chromosome Mol.Biol.Group,
Dept. of Biochemistry,
University of Oxford,
South Parks Road,
Oxford OX1 3QU

Tel:- 01865 275222
____________________________________________________________________________




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