fixation for GFP

Naoaki Saito naosaito at inherit.biosig.kobe-u.ac.jp
Tue Sep 24 04:44:52 EST 1996


Dear Netters,

We express GFP fusion protein in mammalian cells (CHO, COS etc) and obtain
good fluorescence in living cell. But when we fixed with 4%
paraformaldehyde or 10% formalin, the fluorescence was markedly decreased. 
 Do anyone know the good fixative for GFP?  Or please let me know some
references.  

-- 
Nao Saito, Biosignal Research Center, Kobe University, Kobe 657, Japan
e-mail:naosaito at inherit.biosig.kobe-u.ac.jp



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