fura and fluorescent proteins

Xiaoxi Zhuang xz9 at COLUMBIA.EDU
Mon Sep 30 08:32:38 EST 1996


I don't know what you are trying to do, but there's one Ca sensitive
photo protein called aequorin.

On 27
Sep 1996, Cecile Favre wrote:

> 
> I would very much appreciate any comments on the use of mutated
> fluorescent protein (s65t or y66w) together with fura-2
> Would the emission of the GFP photobleach fura?
> What is the percentage of s65t or y66w  emmision at 340 and 380 versus
> fura? 
>  thank you for your help
> 
> Cecile
> cfavre at umabnet.ab.umd.edu 
> 
> 
> 
> 
> 
> 
> 




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