GFP-dimer

vonarnim at UTKUX.UTCC.UTK.EDU vonarnim at UTKUX.UTCC.UTK.EDU
Thu Apr 10 12:53:38 EST 1997


With regard to Beat Ludin's question about the GFP-dimer and its possible
nuclear exclusion:

Yes, I have made a dimer of GFP, and it is indeed excluded from the
nucleus, at least much more efficiently than the monomer.  The dimer is not
twice as bright as the monomer, though.  The trimer was only weakly
fluorescent.  Our assay is a transient expression assay in onion epidermal
cells.

Regards,

Albrecht

Albrecht von Arnim

Dept. of Botany
The University of Tennessee
HBB 437
Knoxville, TN 37996-1100
Phone: (423) 974-6206





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