GFP in rec. plant virus
Dr. George McNamara
george_m at image1.com
Mon Apr 14 09:09:03 EST 1997
Your best options are:
EGFP from Clontech, max fluorescence at about 405 nm, still bright at 490
mGFP/GFP5 from Haseloff. See: K.R. Siemering, R. Golbik, R. Sever, J.
Haseloff (1996) Mutations that suppress the thermostability of green
fluorescent protein. Current Biology 6(12):1653-1663. (see article for
>We are intending to create a recombinant plant virus containing a reporter
>gene in order to locate the progress of infection. Colleagues of us will
>soon buy a fluorescence microscope that we'll be allowed to use, however
>we'll more probably routinely use a hand-held UV lamp.
>Could anyone please help us in the choice of the optimal GFP version to use
>(wt, EGFP, S35T, uv, other...).
>Thanks for your help.
>Olivier Le Gall
> Pathologie Vegetale, INRA Bordeaux, BP 81 :
> 33883 Villenave d'Ornon Cedex, FRANCE -,o
>Tel +33(0)556 843 205 Fax +33(0)556 843 222 ( )
> legall at bordeaux.inra.fr /----"-"---
>* * * * * * *-* *-*-*-*-*-*-*-*-*-*-*-*-----/ W
George McNamara, Ph.D.
Universal Imaging Corporation
502 Brandywine Parkway
West Chester, PA 19380 USA
voice: 610-344-9410 ext 224
More information about the Fluorpro