GFP and Flow Cytometry

Simon D. Scott mlbsds at PICR.CR.MAN.AC.UK
Thu Apr 24 09:02:43 EST 1997


Hello. We're investigating the use of wild-type and mutant GFPs in live 
and fixed cells (e.g. MCF7 breast tumour line) and wish to quantitate 
using FLOW CYTOMETRY. We are counterstaining fixed cells with PI. 70% 
ethanol is the fixative.
 Any advice? - Simon Scott/Brian Marples PICR



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