GFP expression in E. coli

Michael J. Bumbulis mjb10 at po.CWRU.Edu
Fri Apr 25 22:01:33 EST 1997



In a previous article, tremblgu at medcn.umontreal.ca (Guy Tremblay) says:

>Hi,
>
>I am interested in having an E. coli strain transduced with the GFP in
>the chromosome. Anybody has this?
>
>Also, I would like to know if anybody has problems transforming GFP into
>E. coli, on the pGFP plasmid. One student in our lab said he was only
>able to transform it into E.coli when he grew the bacteria on low salt
>agars. That surprises me and I'm curious if someone else encounters this
>problem.

Using standard LB media, I had no problem at all in transforming
E.coli with this plasmid.  
  
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