eGFP deletion

Beat Ludin ludin at FMI.CH
Wed Apr 30 09:57:39 EST 1997


SAINTIGNY Yannick 161011 wrote:

>		Does any one known what will be  the minimal deletion in the 3' end of
>the eGFP coding sequence to knock-out the transcription and/or the
>traduction and/or the  fluorescence of the eGFP, in mammalian cell
>transfected with the peGFP-C3 vector ( without deleting the three STOPs
>codon). What happen if we remove the three last amino-acid of the eGFP ?

As far as I remember (Roger Tsien mentioned it at the last ASCB meeting), 
one can delete a maximum of about 5 AA on the c-terminus of GFP and still 
get a fluorescent protein. The situation should be the same for eGFP.

Beat





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Dr. Beat Ludin, FMI, PO Box 2543, 4002 Basel, Switzerland
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