GFP fusion protein in CHO

Beatrice Marqueze marqueze at jean-roche.univ-mrs.fr
Wed Apr 30 05:06:10 EST 1997


I have constructed a fusion protein with GPF using Clontech vector
pEGFP-N1. My goal is to study in CHO cells the distribution of the fusion
protein in the different sub-cellular compartments.

Does anybody know where GFP alone is distibuted when transfected in CHO, in
the cytoplasm, in the nucleus ? Could the  fusion protein be wrongly
localized in the GFP compartments?


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Beatrice Marqueze-Pouey
INSERM U 374/464, Institut Federatif Jean Roche, Faculte de Medecine-Nord,
13916 Marseille Cedex 20, France.
Tel (33) 4 91 69 88 32, Fax (33) 4 91 09 05 06,
E-mail: marqueze at jean-roche.univ-mrs.fr
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