Clontech's pEGFP-C - NheI site

Roland Brock rbrock at MPC186.MPIBPC.GWDG.DE
Wed Aug 6 08:53:04 EST 1997


I have a question regarding the NheI site that is 5' of the GFP sequence in
Clontechs pEGFP-C vectors. Someone told me that he used Clontechs pS65T-C
vector and its NheI site was gone due to a non-documented point mutation.
Is this an experience that others have made, too? Has anyone successfully
used the NheI site in pEGFP-C vectors for clonings?

Thank you very much for your advise!

Sincerely,

Roland Brock

Roland Brock
Max-Planck-Institute for Biophysical Chemistry
Department of Molecular Biology
Am Fassberg
37077 Goettingen
Germany

Ph.: +49-551-2011772
Fax.: +49-551-2011467
E-mail: rbrock at mpc186.mpibpc.gwdg.de





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