viewing GFP protein on Western blot

Julie R. Hens jrhens at WAM.UMD.EDU
Tue Aug 12 19:07:02 EST 1997


Alex,
Im not positive, but I think that Clontech says that this will not work
regards
julie

On 12 Aug 1997, Alex Chang wrote:

> I am planning to do immunoprecipitation of my GFP-tagged protein, after 
> SDS-PAGE and transblotting onto a membrane, look at the GFP-tagged protein 
> directly by a UV light source without using antibody detection (or simply 
> look at the gel directly without transblotting).
> 
> I'd like to know anyone has done this, and what kind of UV source can be 
> used.
> 
> 
> Alex Chang
> Pathology
> University of British Columbia
> achang at hivnet.ubc.ca
> 
> 




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