Advice about two-color combinations

MP Frosch frosch at dsg.harvard.edu
Wed Aug 20 14:20:13 EST 1997


I am interested in using two different colored forms of GFP as reporters
to compare mutations in a promoter with a "normalization" promoter
signal on a cell-by-cell basis.  What advice or guidance would
experienced users suggest regarding selection of reporters (including
vendors), choice of color ranges and compatibility with commonly
available fluorescence microscopy filters.

TIA,

Matthew Frosch
-- 
M.P. Frosch, MD, PhD

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