general gfp questions

Steffen Dietzel dietzel at sun0.urz.uni-heidelberg.de
Tue Feb 18 03:00:43 EST 1997


In article <33090AEC.BFF at columbia.edu>, ak316 at columbia.edu wrote:

> Dear GFP-ers, 
> 
> I would like to find out a few things about gfp, or the new modified
> versions of gfp such as the ones sold by Clonetech. 
> I plan to express a gfp fusion protein where a nuclear protein is fused
> to the Nterm of gfp. I plan to express this in the mouse and FACS sort
> live cells obtained from such mice.
> 
> 1. does anyone have any experience with FACS sorting of gfp expressing
> cells ? Does it work with mesenchymal embryonic cells ?
> 

The EGFP-Mutatation from Clontech was found by FACS. The procedure is
described in the "Clontechniqes" April 1996, also available at their
Website
(http://www.clontech.com/clontech/Updtoc.html)


> 2. is there a convenient source of gfp DNA that would have the following
> structure:
>         multiple cloning site (MCS)-gfp- poly A addition site - MCS  

I' guess all the commercial products should have that. The structure of
two GFP's  e.g. is described in the same issue of "Clontechniques"



> 
> 3. does this group have a FAQ ? If so, where could i find it ?
> 


Not to my knowledge, but there is the general bionet archive, where you
can find older messages from the newsgroup:
http://www.bio.net:80/hypermail/FLUORESCENT-PROTEINS/

There is also a searchable index.


Greetings

Steffen Dietzel

-- 
Steffen Dietzel
Zentrum fuer molekulare Biologie Heidelberg (ZMBH)
University of Heidelberg
Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Phone: +49/6221/54-6836 or -6879. Fax: -5893
listservmail:dietzel at sun0.urz.uni-heidelberg.de
personal mail: dietzel at wotan.iwr.uni-heidelberg.de



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