general gfp questions
dietzel at sun0.urz.uni-heidelberg.de
Tue Feb 18 03:00:43 EST 1997
In article <33090AEC.BFF at columbia.edu>, ak316 at columbia.edu wrote:
> Dear GFP-ers,
> I would like to find out a few things about gfp, or the new modified
> versions of gfp such as the ones sold by Clonetech.
> I plan to express a gfp fusion protein where a nuclear protein is fused
> to the Nterm of gfp. I plan to express this in the mouse and FACS sort
> live cells obtained from such mice.
> 1. does anyone have any experience with FACS sorting of gfp expressing
> cells ? Does it work with mesenchymal embryonic cells ?
The EGFP-Mutatation from Clontech was found by FACS. The procedure is
described in the "Clontechniqes" April 1996, also available at their
> 2. is there a convenient source of gfp DNA that would have the following
> multiple cloning site (MCS)-gfp- poly A addition site - MCS
I' guess all the commercial products should have that. The structure of
two GFP's e.g. is described in the same issue of "Clontechniques"
> 3. does this group have a FAQ ? If so, where could i find it ?
Not to my knowledge, but there is the general bionet archive, where you
can find older messages from the newsgroup:
There is also a searchable index.
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