Counting GFP colonies
espinoza at cgl.ucsf.edu
Mon Jan 6 17:24:52 EST 1997
pboucher at UMICH.EDU (Paul Boucher) writes:
>I am interested in counting colonies that stably express GFP for
>cytotoxicity assessment studies. I want to be able to count colonies
>that lack and express GFP. Is there an easier way of doing this than
>counting the colonies under a fluorescent microscope? Perhaps some sort
>of fluorescent dish scanner. I would appreciate any input. Thank-you.
Do you need to count colonies, or would counting cells work?
If so, you could use a FACS machine to count up cells expressing and
not expressing GFP. It could give you better numbers and the you
would only have to handle a few tubes instead of many plates.
Well, that's my lame suggestion. -Hernan
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