Globs of GFP fusion in cytosol?

jstrassw at opal.tufts.edu jstrassw at opal.tufts.edu
Tue Jan 7 09:48:12 EST 1997


Hello!
I made a fusion of pEGFP (clontech) to my cDNA of unknown function.  the
resulting pattern is that of 10 or so bright globs in the cytoplasm of HeLa and
C33A cells transiently transfected.  I am concerned that this may represent the
formationm of inclusion bodies due to overexpresession of an insoluble form of
the fusion.  On the other hand, it could represnt targeting to a specific
structure within the cytoplasm.

Thanks for your help,
John
Tufts U.
Boston, USA




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