Counting GFP colonies
scronin at JEEVES.UCSD.EDU
Wed Jan 8 13:20:08 EST 1997
On 6 Jan 1997, Paul Boucher wrote:
> I am interested in counting colonies that stably express GFP for
> cytotoxicity assessment studies. I want to be able to count colonies
> that lack and express GFP. Is there an easier way of doing this than
> counting the colonies under a fluorescent microscope? Perhaps some sort
> of fluorescent dish scanner. I would appreciate any input. Thank-you.
> Laura Zerbe Rubsam
I have been able to look at the fluorescence of yeast colonies with
high levels of s65t gfp on petri plates using a slide projector and an
optical filter for illumination.
scronin at jeeves.ucsd.edu
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