pH-dependence of GFP fluorescence?

Warren Hill whill+ at pitt.edu
Wed Jun 25 12:34:54 EST 1997


Hi

This may be a basic question but have done a quick search and cannot
seem to find any information regarding the pH-dependence of GFP
fluorescence.  I have expressed a fusion protein containing EGFP in Hela
cells and would like to try to measure fluorescence in a cell lysate. 
Does anyone know whether pH is an important parameter in quenching GFP
fluorescence?

Any information greatly appreciated.

Warren Hill
Dept. Cell Biology and Physiology
University of Pittsburgh
whill+ at pitt.edu



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