Fluorimetric measurment of GFP in E.coli

Niklas Gustavsson nbi97ngn at student2.lu.se
Sun Nov 30 07:45:17 EST 1997


Hi there!

Does anyone have a good protocoll for measuring GFP in E.coli? I use a
fluorimeter and the S65T mutation. I'm able to measure GFP from multicopy
plasmids (50-100 copies) but not from a single chromosomal insertion. It's
regulated by a fairly strong promoter. Does anyone have any suggestion?

Regards
Niklas Gustavsson




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