photo for GF colonies

Peter Stroot p-stroot at ux1.uiuc.edu
Fri Oct 17 02:58:27 EST 1997


Soon-Yong Choi wrote:
> 
> Hi!
> 
> When you take a photo for the green fluorescent clolonies (GFPuv vector)
> upon plate, which kind of filter do you use? (plate is irradiated with
> uv365nm hand monitor ). Although I tried many times with yellow or green
> filters, I failed to show colonies colored green.
> 
> Thanks.
> 
> Soon-Yong

this is from a coworker at the Dept. of Animal Sciences at Univ. of
Illinois....

she says to buy a GFP filter set from Chroma Technology Corp.
(Brattleboro, VT, USA) with a Epifluorescence microscope.
also make sure to use the correct wavelength for excitation of the GFP

pete



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