quantifying GFP

Huanfang Zhou hzhou at HSC.USC.EDU
Fri Oct 17 18:59:38 EST 1997


Or, have you heard, there are blue and green GFP vectors from Packard
Instrument Co. (www.packardinst.com), that have signals they claim several
times higher than Clontech GFP and can be measured in a 96-well microplate
in their FluroCount counter. Maybe you can check it out.

H.F. Zhou, Ph.D.

On 17 Oct 1997, Neva Morales wrote:

> HI Todd,
> 
> There is a wonderful article that was published in the the journal of
> microscopy several months ago (I'm sorry I can't remember the volume,
> date, etc. but if you go to the www and search for clontech, they have a
> section specifically for new gfp papers. the paper is quoted there and i
> believe the author's last name is Niswender or something like that).
> 
> to quantify your samples, you might want to make a cell extract and use a
> flurometer. also, someone mentioned to me that flow cytometry is another
> method to quantify your gfp in culture.
> 
> i'll let you know if i come across any articles regarding "quantifying
> gfp." in the meantime, good luck.
> 
> ciao,
> nm
> 
> On 16 Oct 1997, R. Maney wrote:
> 
> > 
> > I know this must be an often asked question but...
> > 
> > I was wondering if someone could clue me in on quantifying GFP expressed
> > in tissue?  All I need to know is relative amounts of GFP expressed. 
> > Can this even be done? 
> > 
> > Thanks
> > 
> > Todd Maney
> > 
> > 
> > 
> > 
> > 
> 
> 
> 




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