Long red shifted GFP?
pirod at pasteur.fr
Tue Oct 21 16:44:08 EST 1997
Damian Purcell <purcell at BURNET.EDU.AU> a écrit dans l'article
> Has anybody heard of a GFP variant (or other fluorescent protein) that
> an excitation maxima around 488nm, but emits in the yellow (580nm) or
> red wavelengths (670nm). The addition of the Blue variant of GFP is
> as a second marker to GFP, but most of the common instrumentation is not
> set up to examine the two excitation and emission wavelengths
> simultaneously. A Yellow or Red fluorescent protein that is stably
> expressed like EGFP and efficiently excited at 488nm would be more useful
> as a second marker with our equipment.
> This is probably like asking for the goose that laid the golden egg!
You are right! EYFP do exist, like EBFP. I saw a paper on Nature a few
months ago (in july or so) describing experiments using EYFP. I don't
remember the vol. sorry.
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