Long red shifted GFP?

[Pierre Rodrigues]

Damian Purcell <purcell at BURNET.EDU.AU> a écrit dans l'article
<v02130500b0689b6627c9@[203.0.141.212]>...
> Has anybody heard of a GFP variant (or other fluorescent protein) that
has
> an excitation maxima around 488nm, but emits in the yellow (580nm) or
long
> red wavelengths (670nm).  The addition of the Blue variant of GFP is
useful
> as a second marker to GFP, but most of the common instrumentation is not
> set up to examine the two excitation and emission wavelengths
> simultaneously.  A Yellow or Red fluorescent protein that is stably
> expressed like EGFP and efficiently excited at 488nm would be more useful
> as a second marker with our equipment.
> 
> This is probably like asking for the goose that laid the golden egg!
> 

You are right! EYFP do exist, like EBFP. I saw a paper on Nature a few
months ago (in july or so) describing experiments using EYFP. I don't
remember the vol. sorry. 

Pierre Rodrigues
Institut Pasteur