quantifying GFP

Pierre Rodrigues pirod at pasteur.fr
Tue Oct 21 16:39:38 EST 1997


> I know this must be an often asked question but...
> 
> I was wondering if someone could clue me in on quantifying GFP expressed
> in tissue?  All I need to know is relative amounts of GFP expressed. 
> Can this even be done? 

If you are working with a cell culture, flow cytometry analysis is the best
way. The intensity of fluorescence will be proportional to the GFP
expression level, if you are using the EGFP variant.

Pierre Rodrigues
Institut Pasteur



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