Downstream translation initiation?

Paul Kitts kitts at NCBI.NLM.NIH.GOV
Thu Dec 31 11:57:03 EST 1998


On 28 Dec 1998 20:05:36 GMT, David N. Levy (levy at uab.edu) wrote:
> One more question: GFP fluoresces fine without the first methionine 
> (which I suppose would be cleaved anyway followoing translation), 
> but does GFP need the valine at position 2 for fluorescence?

David,
GFP does not need the Val at position 2 to fluoresce.   
The second amino-acid in wtGFP is actually Ser:  Met.Ser.Lys...
A Val was inserted in EGFP to create the full Kozak consensus 
sequence around the ATG:  Met.Val.Ser.Lys....
-=Paul=-

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