GFP and Apoptosis

Joachim Seipelt seipelt at
Fri Jan 9 12:03:26 EST 1998

Dear GFPers,

I am expressing a EGFP Fusion protein transiently in mammalian cells and
would like to check whether my
fused protein (or mutants thereof) induce apoptosis or not. Which kind
of assay (preferably in situ) would you recommend  - compatible with GFP
viewing under the microscope to see which cells were transformed?

Many thanks

Dr.Joachim Seipelt
Inst. of Biochemistry
University Vienna
Dr. Bohrgasse 9/3
A 1030 Vienna

Tel.: 43-1-79515-3123
Fax.: 43-1-79515-3114


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