triple labeling

Jordi Barquinero jbarquinero at SUPERMAC.IRO.ES
Mon Jan 12 13:54:14 EST 1998


Dear GFP netters,
We are trying to analyze the simultaneous expression of two phenotypic
markers (CD34 and CD38) in human hematopoietic cells transduced with the
EGFP gene, but EGFP signal (FL1) is very bright and produces a level of
background in the other channels (FL2, FL3 and FL4) that makes color
compensation imposible. We are using a COULTER XL cytometer with an
argon-ion laser (488 nm). Any hints to help us? Thanks.


Jordi Barquinero, M.D.
Institut de Recerca Oncologica
08907 Hospitalet
Barcelona, SPAIN
Tel (93) 2633817
Fax (93) 2632251
jbarquinero at iro.es




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