GFP, BFP, and antibodies

Bratin. K. Saha bsaha at EMORY.EDU
Thu Jan 22 10:50:20 EST 1998


What's the latest on the eukaryotic BFP expression vectors?  Clontech,
Quantum, and any others?  We are interested in labeling two different
proteins, one with GFP (pEGFP works quite well) and the other with BFP, and
then co-express in HeLa.  We want to know if the two will interact and form
a complex.  Will we be able to demonstrate that by microscopy?  What about
FRET?  Did any one try this type of experiment?





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