gorry at burnet.edu.au
Thu Jan 22 19:20:05 EST 1998
<Pine.A22.214.171.1240122163250.43638B-100000 at d1.hrz.uni-giessen.de>, Sandra
Schneider <gf1050 at mailserv.uni-giessen.de> wrote:
> Is there anything known about using GFP expression and indirect
> immunofluorescence simultaneously ?
> Is it possible to fix GFP protein ?
> Thank you for your interest !
I have done this with little success, it appears that GFP loses much of
it's fluorescence, not necessarily with fixation, but with spotting the
cells and drying on slides. Fluorescence can be excellent in wet preps of
fixed cells (acetone or paraformaldehyde).
I hope this helps.
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