photographing GFP colonies from plates

A. S. Goustin Lab hivlab at CMB.BIOSCI.WAYNE.EDU
Fri Jan 30 23:15:51 EST 1998


On Tue, 27 Jan 1998, Brian Robertson wrote:

> Does anyone know how to take pictures of GFP expressing colonies on the UV
> light box?
> What filter is need ?
> What kind of film is best?

Here's a photo I took with Polaroid 667 film and a standard yellow-orange
EtBR filter with a fixed-focus gel documentation camera on a UV lightbox:

     http://cmmg.biosci.wayne.edu/asg/gfplate.gif

The only caveat for this fixed-focus camera is to place spacers under the
camera's hood (cowel) to elevate the camera so that the plane of focus is
~1.5 cm above the transilluminator surface.




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