Fixer

Gregers Gram Gregers.Gram at INET.UNI2.DK
Fri Jul 17 05:30:07 EST 1998


I have used CellFix from Becton&Dickinson. I think it is 2-4% Parafomaldehyde. eGFP
containing cells were analysed after on day one and day 4, only with a few percent
less GFP positive cells.

Gregers
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Gregers J. Gram, cand. scient.
H:S Hvidovre Hospital, Denmark
Email: Gregers.Gram at inet.uni2.dk
3W: http://inet.uni2.dk/home/inflab/gg/
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CHK wrote:

> I am trying to find a way to fix a cell that has been bombarded with a
> GFP/MAP4 construct that is fluorescing for immunolocalization.  The fixer
> has to sustain the fluorescent signal for approx. 12 hrs or more. If you
> know any, please let me know.
>
> C. K.






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