Can we see the green color if we use UV light?

'Mike' Michael J. Moser moser at U.WASHINGTON.EDU
Thu Mar 12 02:05:38 EST 1998


ara

You can easily see GFP fluorescence with a standard transilluminator in
colonies of E. coli expressing GFP from good bacterial promoters.  I don't
know if you can from a mammalian promoter such as CMV. 

Mike Moser                                            Tel: 206-543-6585
UW Department of Pathology                            FAX: 206-543-3967
Box 357705                                       moser at u.washington.edu
Seattle, WA  98195                 http://weber.u.washington.edu/~moser

On Wed, 11 Mar 1998, ara wrote:

> In Clontech catalog, there is a picture, which shows that JM109 cells
> transformed with pEGFP were plated on LB/Amp. The plate was irradiated
> with long-wave UV light for visual detection. Can I see the green color
> of GFP if I use UV-transilluminator?
> If I can do so, what value of wave-length do I need? and is that
> instrument comercial?
> 
> Thanks in advance,
> 
> 
> 
> 




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