.... failed to fluoresce?
Dr. Alex Bogdanov
abogdanov at helix.mgh.harvard.edu
Mon Mar 16 13:04:41 EST 1998
Eugene Kandel. wrote:
>I would suggest to look up the paper or, at least, the abstract
>using it as an example of a failed GFP fusion.
E.Kandel is absolutely right.
T.Aoki et al.
were treating GFP-protein A with heat and observed the loss of
fluorescence and the electrophoretic migration shift. This means that
their fusion is indeed a success (if one measures a success in
fluorescence AU) but a thermolabile one.
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