.... failed to fluoresce?

Dr. Alex Bogdanov abogdanov at helix.mgh.harvard.edu
Mon Mar 16 13:04:41 EST 1998


Eugene Kandel. wrote:


>I would suggest to look up the paper or, at least, the abstract
>before
>using it as an example of a failed GFP fusion.

E.Kandel is absolutely right.  

       T.Aoki et al.  

were treating GFP-protein A with heat and observed the loss of
fluorescence and the electrophoretic migration shift. This means that
their fusion is indeed a success (if one measures a success in  
fluorescence AU) but a thermolabile one.

My apologies...



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