in situ hybridisation

Giovanna Bermano G.Bermano at rri.sari.ac.uk
Tue Mar 17 07:59:06 EST 1998


Hi all,

Has anyone looked at GFP mRNA localization in transfected mammelian cells by 
in situ hybridisation? If so, has the mRNA a particular localization in the 
cells?
I would like to use GFP as a reporter to study the role of 3'UTRs in mRNA 
localization therefore I would like to know if GFP mRNA has a particular 
localization in the cell.
Thank you in advice for any idea or hint

Giovanna

********************************************************************
Dr. Giovanna Bermano                 Tel. 01224 712751 ext. 3255
Rowett Research Institute            Fax. 01224 716622
Greenburn Road                       E-mail. gb at rri.sari.ac.uk
Bucksburn, Aberdeen (UK)
AB21 9SB




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