Anti-Fade for GFP

'Mike' Michael J. Moser moser at U.WASHINGTON.EDU
Fri Mar 27 14:12:40 EST 1998


Steffen

Try Citifluor Glycerol from Ted Pella.  I have also heard that "Elviol"
(sp?) from Molecular Probes works well.

Mike Moser                                            Tel: 206-543-6585
UW Department of Pathology                            FAX: 206-543-3967
Box 357705                                       moser at u.washington.edu
Seattle, WA  98195                 http://weber.u.washington.edu/~moser

On Thu, 26 Mar 1998, Steffen Dietzel wrote:

> Hi,
> 
> I am looking at mammal cell culture cells expressing GFP. To facilitate
> observation of cells in the fluorescent microscope, I would like to use an
> Anti-Fade reagent that minimizes bleaching. Currently we have Propyl Galate
> solved in 90% Glycerol in the lab, however it quenches the GFP-fluorescene in
> formaldehyde fixed cells pretty much!
> 
> Does anybody know something better than that for fixed cells? Since I am
> intersted in fine structure, my gut-feeling is not to try hardening embeding
> media, because it might deform 3D structure during the hardening process. 
> 
> Also, if somebody would know something for living cells, this also would be
> most interesting.
> 
> Thanks for all replies
> 
> Steffen
> 
> 




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