Anti-Fade for GFP

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Mar 27 14:54:52 EST 1998


Steffen Dietzel <s.dietzel at pop.life.uiuc.edu> wrote:
> I am looking at mammal cell culture cells expressing GFP. To facilitate
> observation of cells in the fluorescent microscope, I would like to use an
> Anti-Fade reagent that minimizes bleaching. Currently we have Propyl Galate
> solved in 90% Glycerol in the lab, however it quenches the GFP-fluorescene in
> formaldehyde fixed cells pretty much!

Are you sure that it is the Propyl Galate? When I mount GFP-expressing
cells in Propyl Galate without fixing, I get a lot of fluorescence;
however, when I first fix them in 4% paraformaldehyde, fluorescence is
almost completely lost :-(

Any hints appreciated...

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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