(none)

[Chris Lau]

We tried the same IRES-EGFP vector in HeLa cells and saw real good EGFP
expression, approximately 50% cells were fluorescent.  We used BRL
TransfectAMINE plus kit for our transient transfection experiment.  EGFP
expression was detectable from 24 -76 hours after addition of DNA with the
same % of positive cells. 

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On 28 Mar 1998, Erick Noensie wrote:

> I am using the Clontech IRES-EGFP vector.  I know that the IRES is
> attenuated, but I can't see anything glowing in Hela or mouse cells. I also
> did controls for transfection so I dont think thats the problem.  Has
> anyone else had this problem or have any insight into this ? 
> 
> Thanks.
> 
> 
> 
> Erick Noensie
> Johns Hopkins University School of Medicine
> 
> 
> 

_____________________________________________________________________________

Chris Lau, Ph.D. 				Tel: 415 476-8839 
Division of Cell and Developmental Genetics 	FAX: 415 502-1613 
Department of Medicine, VAMC-111C5 
University of California, San Francisco 	E-mail : clau at itsa.ucsf.edu 
4150 Clement Street
San Francisco, CA 94121