anti-GFP Abs

DENISE at cellbio.emory.edu DENISE at cellbio.emory.edu
Mon Oct 19 13:13:32 EST 1998


To:            fluorpro at net.bio.net
From:          lapointr at post.queensu.ca (Renee Lapointe)
Subject:       EGFP sensitivity augmentation by anti-GFP Abs
Date:          14 Oct 1998 12:43:11 -0700

Hello everyone,
I have two questions for the group.

1) Therefore we would like to know whether the use of a
GFP specific antibody could improve the signal. Has anyone tested that?

*Yes, I've tested Chemicon's chicken anti-GFP with good success.  I've been able to use it at 
*a 1:5000 dilution in immunohistochemistry, and at around 1:20,0000 dilution on western 
*blots.  This may be a better bet for you, given question #2 as well.

2) I guess this brings me to the second part of my message. CLONTECH, the
company which sells these vectors, also sells a polyclonal antibody against
GFP, but it's titer for immunofluorescence is 1/200, rendering the cost of
its use too high for us to use (we have numerous promoters and conditions
to test). Does anyone knows of a cheaper source of GFP-specific antibody
which would detect EGFP even in a denatured form (for westerns for example)?

Thank you for your help,

Renee Lapointe

Rene Lapointe
Department of Microbiology and Immunology
Queen's University, Kingston, Ontario, K7L 3N6
CANADA
Tel.:   (613) 545-6702, FAX: (613) 545-6796
E-mail: lapointr at post.queensu.ca







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