Weidong Xie wrote in message <37ADDB6B.B43644EE at stjude.org>... >Hi, I am trying to fusion antibodies with GFP, Is this protein working >in vitro, or any one else have tried this before?? It does fluoresce in vitro. The problem you might get is sensitivity since GFP is not based on enzymatic activity. But since its in vitro, one just leaves it on the film longer.