immunofluorescence

m.hagedorn m.hagedorn at auckland.ac.nz
Wed Aug 18 18:52:20 EST 1999


Hi there
I am doing colocalization of a GFP-tagged protein and using
immunofluorescence.
I found that after permeabilizing the cells with 0.1% tritonX100 in
1xPBS the GFP-signal disappears or weakens.
Did anyone have the same observation or can help me?
Thanks




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