Human and electronic dynamic ranges for protein spot intensities

Douglas Kell dbk at aber.ac.uk
Mon Aug 30 04:36:22 EST 1999


1. Modern CCD cameras used in gel readers have a 16-bit dynamic range (or at
least save images as 16-bit tiff files)..
2. The human eye has a dynamic range for grey scale in a given pixel of not
more than 7 bits.
3. Conclusion: there is a lot more info in a gel than the eye can see, but
the processing we'd do on a computer etc to improve the image content or
dynamic range of an analysis does not  show up in a way that would a priori
persuade someone looking at a picture.

How do people deal with this? Can replies be cc'd to my mailbox as well as
this group?

Many thanks,
Douglas.
--
(Prof.) Douglas Kell
Inst. Biol. Sci., Cledwyhn Bldg,
UWA, Aberystwyth SY23 3DD, UK
dbk at aber.ac.uk    http://gepasi.dbs.aber.ac.uk





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