Detecting GFP expression driven by mammalian promoters

[Eugene Kandel]

You are right. I analyzed cells in PBS.

"'Mike' Michael J. Moser" wrote:
> 
> On Tue, 9 Feb 1999, Eugene Kandel wrote:
> ...
> > Before reading I substituted medium for PBS, but I do not know how
> > important it is.
> ....
> 
> Eugene
> 
> Do you mean substituted PBS for medium?  I figured GFP would be harder to
> see in the higher fluorescent background generated by medium.
> 
> Thanks much for the advice!
> 
> Mike
> 
> Mike Moser, Ph.D.                                     Tel: 206-543-6585
> UW Department of Pathology                            FAX: 206-543-3967
> Box 357705                                       moser at u.washington.edu
> Seattle, WA  98195                 http://weber.u.washington.edu/~moser

-- 
Dr Eugene S. Kandel
The University of Illinois at Chicago 
Department of molecular Genetics (m/c 669)
900 S. Ashland Ave, Rm 2106
Chicago IL, 60607

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