GFP in protein location

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Nov 2 05:02:29 EST 1999


Liao Cheng <chliao at online.sh.cn> wrote:
> Would I fuse my protein into the N-terminal or C-terminal of
> GFP? Which will be better?

You have to try it for yourself. Some proteins will tolerate N-terminal
fusions better than C-terminal ones, others vice versa and still others
won't tolerate either.

As for making the fusion, it's just ordinary molecular biology - no
special protocols needed.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */




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