myc-6xHis-tag on GFPuv

Matthias Paschke mpaschke at charite.de
Fri Nov 12 04:44:46 EST 1999


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i made a construct of GFPuv carrying a c-terminal Myc-6xHis-tag, but i
can not detect any fluorescence when it is expressed in e.coli (XL-1).
does anyone knows something about negativ effects of c-terminal
tagged-GFP opon expressonlevels and/or fluorescence. It seems like most
people are using an n-terminal 6xHis-tagged constructs for bacterial
expression.
thanks for your help, matthias

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